Blood sampling is generally applied to provide more insight in metabolic regulation and to allow characterisation of subject/patient populations. Depending on the setting blood is obtained by fingerprick, venous needle sampling, venous catheter sampling, arterialised blood sampling (hot-box approach), deep venous and/or arterial blood sampling. Blood and/or plasma samples are collected, processed and stored appropriately, depending on the specific analyses.
The analytical methods we use for plasma plasma analyses include fluorimetric and enzymatic assays performed on the Cobas-Fara, –Mira and -Pentra like glucose, LDH, lactate, free fatty acids, glycerol, triglycerides, ethanol, creatinin, acetate, calcium, BCAA, glycogen, cholesterol (HDL, LDL and VLDL), Apo A1 and B, albumin and ATP. Insulin, glucagon, and various adipokine concentrations are generally performed with RIA (and/or ELISA), plasma isotope enrichment analyses by GC-(IR)MS. Catecholamine concentrations, blood HbA1c contents, plasma FFA profiles, and amino acid concentrations are generally performed using HPLC.
Besides the infrastructure at the Departments of Human Biology and Human Movement Sciences, there is additional support from various other labs within the University, the Academic Hospital (Clinical Chemistry Department) and external labs. Consequently, the options for specific blood and/or plasma analyses are unrestricted.